The local lymph node assay (LLNA), as recommended by the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), only allows for the use of CBA mice. The objective of these studies was to begin to assess the response of chemical sensitizers in the LLNA across six strain
Dietary vitamin A enhances sensitivity of the local lymph node assay
โ Scribed by Denise M. Sailstad; Sonali D. Krishnan; Jeffrey S. Tepper; Donald L. Doerfler; MaryJane K. Selgrade
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 703 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0300-483X
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## Abstract The local lymph node assay (LLNA) is a skin sensitization test that provides animal welfare benefits. To reduce animal usage further, a modified version (rLLNA) was proposed. Conducting the rLLNA as a screening test with a single high dose group and vehicle control differentiated accura
A modified version of the local lymph node assay (LLNA) is presented, using bromodeoxyuridine to label proliferating lymphocytes. Cell counting is done on mid-sagittal sections of individual nodes under light microscopy. Two irritants (sodium lauryl sulfate and salicylic acid), four allergens of var
The murine local lymph node assay is a predictive method for the identification of skin-sensitizing chemicals in which activity is measured as a function of proliferative activity induced in lymph nodes draining the site of exposure. In the present study, the induction by topically applied chemicals