Diagnosis of gastric cancers with fluorescein-labeled monoclonal antibodies to carcinoembryonic antigen

✍ Scribed by Masaharu Tatsuta; Hiroyasu Iishi; Makoto Ichii; Miyako Baba; Reiko Yamamoto; Shigeru Okuda; Katsuya Kikuchi

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 790 KB
• Volume: 9
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.1900090415

✦ Synopsis

Forty gastric adenocarcinomas in 30 resected stomachs were examined by an endoscopic immunofluorescent technique using fluorescein isothiocyanate (FITCI-labeled antibodies to carcinoembryonic antigen (CEA). Fluorescence images of the tumors were obtained with a newly developed endoscopic television system for detecting faint fluorescence. Computer-assisted processing was used to enhance the detection and localization of the tumors visualized by the immunofluorescent technique. Twenty seven (90%) of 30 tumors showed positive fluorescence after treatment with FITC-labeled antibodies for 60 minutes, whereas only two (40%) of five cancers could be detected by the immunofluorescent technique after treatment with antibodies for less than 60 minutes. There was no significant relationship between positive fluorescence and the gross and histological types or the stages of the tumors. In contrast, no positive fluorescence could be demonstrated in cases of benign gastric lesions or after pretreatment with FITC-labeled alpha-fetoprotein. This study therefore suggests that the immunofluorescent technique using FITC-labeled anti-CEA antibodies can be useful in detecting the early stages of gastric cancer.