๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Development of an enzyme-based assay for acetaminophen

โœ Scribed by Peter M. Hammond; Michael D. Scawen; Tony Atkinson; R.Stewart Campbell; Christopher P. Price

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
437 KB
Volume
143
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A new and novel method for determination of serum acetaminophen is described. The assay, which can be completed in less than 5 min, is based on the enzymatic hydrolysis of acetaminophen, with subsequent calorimetric detection of the aminophenol so produced. Various possible means of aminophenol estimation are described, the final reaction conditions have been optimized for maximum sensitivity and assay speed. This assay compares favorably with other avai l abl e procedures; it requires only small sample volumes; it is rapid, simple, and highly specific for the parent drug; and it requires neither great technical ability nor expensive hStfUlllell~tiOll.

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