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Enzyme electrode-based kinetic assays of enzyme activities

✍ Scribed by C.R. Gebauer; M.E. Meyerhoff; G.A. Rechnitz

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 303 KB
Volume: 95
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90759-0

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✦ Synopsis

Potentiometric enzyme electrodes for urea, AMP, and adenosine have been used for the kinetic assays of arginase (EC 3.5.3.1), 3':5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17), and 5'-nucleotidase (EC 3.1.3.5), respectively. The initial rate of potential change, after addition of the enzyme to the substrate solution, is directly proportional to the enzyme activity present. Arginase assays were found to be reproducible to a relative precision of 13% or better and applicable to the direct measurement of arginase activity in beef liver homogenates.

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