𝔖 Bobbio Scriptorium
✦   LIBER   ✦

An immunospecific enzyme assay for horseradish peroxidase

✍ Scribed by L. Ambler; G.E. Peters

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
204 KB
Volume
137
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A sensitive assay highly specific for horseradish peroxidase has been developed. The basic principle of the procedure is similar to that of the enzyme-linked immunosorbent assay technique. Sensitivity is the range 0.156-2.5 ng/ml. Background activity in undiluted plasma and lymph under these conditions is negligible.

πŸ“œ SIMILAR VOLUMES

Photochemical amplification for horserad
Photochemical amplification for horseradish peroxidase-mediated immunosorbent assay
✍ Semion M. Bystryak; Vladimir M. Mekler πŸ“‚ Article πŸ“… 1992 πŸ› Elsevier Science 🌐 English βš– 405 KB

A new method for lowering the detection limit for a horseradish peroxidase (HRP) label in an enzyme-linked immunosorbent assay (ELISA) is proposed. The method is based on the use of a photochemical reaction of o-phenylenediamine (o-PD) autosensitized oxidation as an enhancement step in ELISA. The as

A Bifunctional Luminogenic Substrate for
A Bifunctional Luminogenic Substrate for Two Luminescent Enzymes: Firefly Luciferase and Horseradish Peroxidase
✍ T. Sudhaharan; A.Ram Reddy πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 107 KB

Horseradish peroxidase (HRP) catalyzes the oxidative chemiluminescent reaction of luminol, and firefly luciferase catalyzes the oxidation of firefly D-luciferin. Here we report a novel substrate, 5-(5-azoluciferinyl)-2,3-dihydro-1,4-phthalazinedione (ALPDO), that can trigger the activity of HRP and

An enzymic assay for ribitol
An enzymic assay for ribitol
✍ D.R.D. Shaw; David Mirelman πŸ“‚ Article πŸ“… 1970 πŸ› Elsevier Science 🌐 English βš– 150 KB