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Development of a transformation system for the multinuclear yeastDipodascus (Endomyces) magnusii

✍ Scribed by Adamíková, L'ubica; Griač, Peter; Tomáška, L'ubomír; Nosek, Jozef

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
340 KB
Volume
14
Category
Article
ISSN
0749-503X

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✦ Synopsis

We have developed the first system for genetic transformation of the multinuclear yeast Dipodascus magnusii. The system is based on a dominant selectable marker and an autonomously replicating sequence. We have constructed a plasmid vector which contains a marker conferring resistance to zeocin and the segment of non-transcribed spacer of D. magnusii ribosomal DNA which supports the autonomous replication of plasmid DNA in yeast cells. Plasmid DNA has been transferred into D. magnusii cells by electroporation.

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We present here the first report of a transformation system developed for the filamentous, ML-236B (compactin)-producing fungus Penicillium citrinum. Hygromycin B-resistant colonies were obtained after treatment of protoplasts with a vector containing an Escherichia coli hygromycin B phosphotransfer