𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Development of a homologous transformation system for Aspergillus niger based on the pyrG gene

✍ Scribed by van Hartingsveldt, Wim ;Mattern, Ineke E. ;van Zeijl, Cora M. J. ;Pouwels, Peter H. ;van den Hondel, Cees A. M. J. J.


Publisher
Springer
Year
1987
Tongue
English
Weight
607 KB
Volume
206
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Transformation of Aspergillus oryzae usi
✍ Mattern, Ineke E. ;Unkles, Shiela ;Kinghorn, Jim R. ;Pouwels, Peter H. ;van den πŸ“‚ Article πŸ“… 1987 πŸ› Springer 🌐 English βš– 248 KB

A transformation system for Aspergillus oryzae based on the orotidine-5'-phosphate decarboxylase gene (pyrG) was developed. Transformation frequencies of up to 16 transformants per microgram of DNA were obtained with the vector pAB4-1, which carries the pyrG gene of A. niger. Southern blotting analy

Development of a homologous transformati
✍ Horng, Jason S. ;Chang, Perng-Kuang ;Pestka, James J. ;Linz, John E. πŸ“‚ Article πŸ“… 1990 πŸ› Springer 🌐 English βš– 363 KB

The nitrate reductase structural gene (niaD) and an niaD mutant strain were isolated from Aspergillus parasiticus and used to develop a homologous transformation system. A transformation frequency of 110 to 120 transformants per microgram linear DNA was obtained with the 10.9 kb plasmid pSL82, which