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The development of a homologous transformation system for Aspergillus oryzae based on the nitrate assimilation pathway: A convenient and general selection system for filamentous fungal transformation

โœ Scribed by Unkles, Shiela E. ;Campbell, Edward I. ;de Ruiter-Jacobs, Yolanda M. J. T. ;Broekhuijsen, Martien ;Macro, Janet A. ;Carrez, Dirk ;Contreras, Roland ;van den Hondel, Cees A. M. J. J. ;Kinghorn, James R.


Publisher
Springer
Year
1989
Tongue
English
Weight
675 KB
Volume
218
Category
Article
ISSN
0026-8925

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Development of a homologous transformati
โœ Horng, Jason S. ;Chang, Perng-Kuang ;Pestka, James J. ;Linz, John E. ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 363 KB

The nitrate reductase structural gene (niaD) and an niaD mutant strain were isolated from Aspergillus parasiticus and used to develop a homologous transformation system. A transformation frequency of 110 to 120 transformants per microgram linear DNA was obtained with the 10.9 kb plasmid pSL82, which