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Development of a serum-free and heat-sterilizable medium and continuous high-density cell culture

✍ Scribed by Yoshiki Minamoto; Koji Ogawa; Hideki Abe; Yoko Iochi; Koji Mitsugi

Publisher
Springer
Year
1991
Tongue
English
Weight
1018 KB
Volume
5
Category
Article
ISSN
0920-9069

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## Abstract The steady‐state metabolic parameters for a hybridoma cell line have been determined in continuous suspension–perfusion culture over a wide range of perfusion rates and cell bleed rates. Significant increases in viable cell concentrations and volumetric productivities were achieved at h