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Development of a Recombinant Bacterial Expression System for the Active Form of a Human Transforming Growth Factor β Type II Receptor Ligand Binding Domain

✍ Scribed by Christian C. Boesen; Shawn A. Motyka; Apisit Patamawenu; Peter D. Sun

Book ID
115645724
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
212 KB
Volume
20
Category
Article
ISSN
1046-5928

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✍ Mark S. Fahey; David Dawbarn; Shelley J. Allen; Ian C. Paterson; Stephen S. Prim 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 87 KB

TGF-␤ is a ubiquitous protein that exhibits a broad spectrum of biological activity. The prokaryotic expression and purification of the extracellular domain of the type II TGF-␤ receptor (T␤R-II-ED), without the need for fusion protein cleavage and refolding, is described. The recombinant T␤R-II-ED