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Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus

✍ Scribed by Theresa Cromeans; Mark D. Sobsey; Howard A. Fields

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
851 KB
Volume
22
Category
Article
ISSN
0146-6615

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✦ Synopsis

Most hepatitis A virus (HAV) replication in cell culture has been reported to be nonlytic and relatively slow. A rapidly replicating isolate of strain HM-175 from persistently infected, serially passed cell cultures (pHM-175) was found to induce a cytopathic effect. This observation allowed the development of a classic plaque assay for pHM-175 in FRhK-4 cells. The plaques were neutralized by polyclonal and monoclonal antisera to HAV.

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