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Cytopathology, plaque assay, and heat inactivation of hepatitis A virus strain HM175

✍ Scribed by David A. Anderson

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
784 KB
Volume
22
Category
Article
ISSN
0146-6615

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✦ Synopsis

Hepatitis A virus (HAV) strain HM175 derived after repeated subculture of persistently infected B-SC-1 cells caused a specific cytopathic effect upon acute infection of B-SC-1 cells. The virus formed visible plaques on B-SC-1 cell monolayers after 9 to 14 days of incubation at 34 degrees C, and virus can therefore be titrated by plaque assay. Virus could be re-isolated from plaques of infected cells, which allows the clonal isolation of HAV variants. The stability of a plaque-purified variant of HAV at elevated temperatures exceeded that of poliovirus type 1, but this variant is less stable than previously reported strains of HAV.

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