𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Development of a hepatitis delta virus antibody assay for study of the prevalence of HDV among individuals infected with hepatitis B virus in China

✍ Scribed by Liping Shen; Ying Gu; Le Sun; Yingchao Yang; Feng Wang; Yimin Li; Shengli Bi

Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
83 KB
Volume
84
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Co‐infection with hepatitis delta virus (HDV) and hepatitis B virus (HBV) has been shown to be associated with a more severe form of acute and chronic hepatitis. Cloning and expression of recombinant HDV antigen (rHDAg) in Escherichiacoli are described. Using purified rHDAg, a cost‐effective indirect anti‐HDV enzyme‐linked immunosorbent assay (ELISA) kit was developed. Direct comparison of 15 known HDV‐positive sera and 15 HDV‐negative sera showed concordance agreement between the new assay kit and the Abbott Murex Anti‐Delta (total) kit. In addition, 1,486 hepatitis B surface antigen (HBsAg) positive blood samples collected from various areas of China were tested using this indirect anti‐HDV ELISA. It was found that 1.2% (95% CI: 0.7–1.9%) of the samples were anti‐HDAg positive. It is suggested that the prevalence of HDV and HBV co‐infection in China is relatively low. J. Med. Virol. 84:445–449, 2012. © 2011 Wiley Periodicals, Inc.

📜 SIMILAR VOLUMES

Prevalence of Hepatitis C Virus Infectio
Prevalence of Hepatitis C Virus Infection in Hemodialysis Patients: A Longitudinal Study Comparing the Results of RNA and Antibody Assays
✍ Tak Mao Chan; Anna Suk Fong Lok; Ignatius Kum Po Cheng; Rachel Tzun Chan 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 465 KB 👁 1 views

We longitudinally studied 51 patients from two hemodialysis centers to determine the prevalence of hepatitis C virus infection in hemodialysis patients. Serum samples were tested for antibody to HCV by first-and second-generation enzyme immunoassays and for hepatitis C virus RNA by nested polymerase

Prevalence of antibodies to hepatitis E
Prevalence of antibodies to hepatitis E virus among apparently healthy humans and pigs in Bali, Indonesia: Identification of a pig infected with a genotype 4 hepatitis E virus
✍ I. Dewa Nyoman Wibawa; David H. Muljono; Mulyanto; I.G.A. Suryadarma; Fumio Tsud 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 105 KB 👁 1 views

## Abstract In Indonesia where hepatitis E virus (HEV) is believed to be highly endemic, only three outbreaks of HEV transmission have been documented to date in restricted areas (West Kalimantan and East Java). A total of 1,115 serum samples collected from apparently healthy individuals in Bali, L

Infection of B cells with hepatitis C vi
Infection of B cells with hepatitis C virus for the development of lymphoproliferative disorders in patients with chronic hepatitis C
✍ Momoko Inokuchi; Takayoshi Ito; Manabu Uchikoshi; Yuu Shimozuma; Kenichi Morikaw 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 214 KB 👁 1 views

## Abstract Infection with hepatitis C virus (HCV) is associated with lymphoproliferative disorders, represented by essential mixed cryoglobulinemia and B‐cell non‐Hodgkin's lymphoma, but the pathogenic mechanism remains obscure. HCV may infect B cells or interact with their cell surface receptors,

Prevalence of antibody to the hepatitis
Prevalence of antibody to the hepatitis A virus in a large institution for the mentally retarded
✍ Noreen I. Lehmann; D. L. B. Sharma; Dr. Ian D. Gust 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 English ⚖ 308 KB 👁 1 views

The prevalence of antibody to hepatitis A virus (anti-HAV) was determined in 854 people living in a large institution for the mentally retarded in Melbourne. Altogether 638 (74.4%) of the subjects were found to have specific antibody detectable by solid-phase radioimmunoassay (SPRIA). No difference

Neutralization of Hepatitis B Virus Infe
Neutralization of Hepatitis B Virus Infectivity by a Murine Monoclonal Antibody: An Experimental Study in the Chimpanzee
✍ Dr. Sten Iwarson; Edward Tabor; Howard C. Thomas; Allison Goodall; Jenny Waters; 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 440 KB 👁 1 views

## Abstract Two study chimpanzees were inoculated intravenously with approximately 1,000 chimpanzee infectious doses of hepatitis B virus (HBV), one with subtype adr and one with subtype ayw, each previously incubated with 0.1 ml of a murine monoclonal antibody (IgG ~1(K)~ class) directed against a

Age-specific prevalence and significance
Age-specific prevalence and significance of hepatitis B e antigen and antibody in chronic hepatitis B virus infection in taiwan: A comparison among asymptomatic carriers, Chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma
✍ Yun-Fan Liaw; Chia-Ming Chu; Deng-Yn Lin; I-Shyan Sheen; Chaur-Young Yang; Miau- 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 434 KB 👁 1 views

The age-specific prevalence of hepatitis B e antigen (HBeAg) and its antibody (anti-HBe) were studied by radioimmunoassay, and compared in a large series of patients with chronic hepatitis B virus (HBV) infection, including 268 asymptomatic carriers, 389 chronic hepatitis, 114 liver cirrhosis, and 2