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Development of a new culture system for human lymphokine-activated killer cells

✍ Scribed by Mitsuhiro Murata; Tokujiro Yano; Masatoshi Togami; Kosei Yasumoto; Keizo Sugimachi; Genki Kimura; Kikuo Nomoto

Book ID
118902073
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
495 KB
Volume
129
Category
Article
ISSN
0022-1759

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Development of a new culture system for
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We recently developed a new culture system based on dialysis perfusion (designated JCC-device) for the generation and expansion of human lymphokine-activated killer (LAK) cells (Murata et al., 1990). More recently we have scaled up the volume of the culture vessel of the JCC-device from 100 ml to 40

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In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conv