✦ LIBER ✦

A new high-yield continuous cell-culture system for lymphokine-activated killer cells

✍ Scribed by Takashi Noto; Yutaka Tokuda; Yoshihiko Nakamura; Akira Suzuki; Katsuto Watanabe; Masaichi Yamamura; Tomoo Tajima; Toshio Mitomi; Kazuhiro Nishijima

Publisher: Springer-Verlag
Year: 1989
Tongue: English
Weight: 629 KB
Volume: 30
Category: Article
ISSN: 0340-7004
DOI: 10.1007/bf01665023

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Generation of lymphokine activated kille

Generation of lymphokine activated killer cells in a new high density dialyzing culture apparatus

✍ Yutaka Kohgo; Sumio Sakamaki; Yuji Kanisawa; Shuichi Nojiri; Yoshinori Ueno; Yos 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 570 KB

In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conv

Culture supernatants of lymphokine-activ

Culture supernatants of lymphokine-activated killer (LAK) cells contain a high-molecular-weight cytotoxic lymphokine

✍ Sarit Larisch-Bloch; Barbara Sterman; David Weiss; Dov Sulitzeanu 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 265 KB

Development of a new culture system for

Development of a new culture system for human lymphokine-activated killer cells: Comparison with a conventional static culture method

✍ Mitsuhiro Murata; Tokujiro Yano; Ichiro Yoshino; Masatoshi Togami; Masateru Soga 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 567 KB

We recently developed a new culture system based on dialysis perfusion (designated JCC-device) for the generation and expansion of human lymphokine-activated killer (LAK) cells (Murata et al., 1990). More recently we have scaled up the volume of the culture vessel of the JCC-device from 100 ml to 40

A high molecular weight cytotoxic lympho

A high molecular weight cytotoxic lymphokine in supernatants of lymphokine-activated killer cells cross-reacts with transforming growth factor beta

✍ Sarit Larisch-Bloch; Barbara Sterman; Dov Sulitzeanu 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 308 KB

High-density continuous cultures of hybr

High-density continuous cultures of hybridoma cells in a depth filter perfusion system

✍ Duk Jae Oh; Sang Kyo Choi; Ho Nam Chang 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 637 KB

A high-yielding, generic fed-batch cell

A high-yielding, generic fed-batch cell culture process for production of recombinant antibodies

✍ Paul W. Sauer; John E. Burky; Mark C. Wesson; Heather D. Sternard; Limin Qu 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 690 KB

A fed-batch cell culture process was developed that has general applicability to all evaluated Sp2/0 (n = 8) and NS0 (n = 1) antibody-producing cell lines. The two key elements of this generic process were a protein-free concentrated feed medium, and a robust, metabolically responsive feeding strate