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Generation of lymphokine activated killer cells in a new high density dialyzing culture apparatus

โœ Scribed by Yutaka Kohgo; Sumio Sakamaki; Yuji Kanisawa; Shuichi Nojiri; Yoshinori Ueno; Yoshinori Itoh; Minoru Takahashi; Yutaka Sasagawa; Shinji Hosoi; Seiji Sato; Yoshiro Niitsu

Publisher
Springer
Year
1989
Tongue
English
Weight
570 KB
Volume
2
Category
Article
ISSN
0920-9069

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โœฆ Synopsis

In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture conditions. By using this new apparatus with continuous regulation of infused interleukin 2, nutritional medium, and pO(2) and pCO(2), yields of 2ร—10(7) cells/ml were achieved and maintained for more than 21 days. These cells also showed a significant increase of LAK activity on a per cell basis.

๐Ÿ“œ SIMILAR VOLUMES

Development of a new culture system for
Development of a new culture system for human lymphokine-activated killer cells: Comparison with a conventional static culture method
โœ Mitsuhiro Murata; Tokujiro Yano; Ichiro Yoshino; Masatoshi Togami; Masateru Soga ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 567 KB

We recently developed a new culture system based on dialysis perfusion (designated JCC-device) for the generation and expansion of human lymphokine-activated killer (LAK) cells (Murata et al., 1990). More recently we have scaled up the volume of the culture vessel of the JCC-device from 100 ml to 40