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Development of a nano-electrospray mass spectrometry source for nanoscale liquid chromatography and sheathless capillary electrophoresis

✍ Scribed by James N. Alexander IV; Gary A. Schultz; Janet B. Poli

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 226 KB
Volume: 12
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19980915)12:17<1187::aid-rcm300>3.0.co;2-t

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✦ Synopsis

A Fisons Quattro I electrospray ionization (ESI) source has been modified to produce stable electrospray ion currents at flow rates as low as 50 nL/min. The original counter electrode and skimmer cone lens of the Fisons ESI source have been replaced with a spherical cone lens. This improved source provides a greater range of x,y,z positioning of a stainless steel tip resulting in a stable ion signal for flow rates of 50 nL/min to 2 mL/min. A tapered stainless steel electrospray tip (50 mm i.d.) was evaluated for mass spectrometry using nano-liquid chromatography (50 mm i.d., flow rate = 120 nL/min) and sheathless capillary electrophoresis. The analysis of a nonionic surfactant, octylphenol ethoxylate, was accomplished with both nanoscale separation techniques.

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