✦ LIBER ✦

Development of a gas chromatographic/mass spectrometric method to quantify R(–)-apomorphine, R(–)-apocodeine and R(–)-norapomorphine in human plasma and urine

✍ Scribed by Frédéric Libert; François Coudoré; Damien Richard; Franck Durif; Alain Eschalier

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 119 KB
Volume: 40
Category: Article
ISSN: 1076-5174
DOI: 10.1002/jms.939

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A method was developed and validated for the analysis of R(−)‐apomorphine, (R−)‐apocodeine and R(−)‐norapomorphine in human plasma and urine with N‐propylnorapomorphine as internal standard using gas chromatography/mass spectrometry (GC/MS) and single‐ion monitoring after a single liquid–liquid extraction and silylation of compounds. The quantification limits were 1 ng/ml for apomorphine and apocodeine and 25 ng/ml for norapomorphine. Calibration curves were linear, within the range 1–100 ng/ml. Variation in intraday and interday precision was below 10%. This method was applied to study apomorphine bioavailability in nine patients with Parkinson's disease before and after coadministration of a catechol‐O‐methyl transferase inhibitor. Copyright © 2005 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Validation of a gas chromatographic/mass

Validation of a gas chromatographic/mass spectrometric method for the determination of (E)-β-fluoromethylene-m-tyrosine and its active metabolite in human plasma and urine

✍ Josiane Schoun; Bertrand Duléry; Klaus D. Haegele 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 461 KB 👁 2 views

Fhoromethylene-rn-tyrosine (FMMT, MDL 72394) represents a prodrug approach to site-selective, irreversible inhibition of monoamine oxidase. A sensitive and specific method for the quantification of FMMT and its active metabolite (MDL 72392) in human plasma and urine has been developed for future pha

Quantitative gas chromatographic mass sp

Quantitative gas chromatographic mass spectrometric determination of pinaverium—bromide in human serum

✍ G. A. de Weerdt; R. P. Beke; H. G. Verdievel; F. Barbier; J. A. Jonckheere; A. P 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 427 KB 👁 2 views

A sensitive gas chromatographic mass spe

A sensitive gas chromatographic mass spectrometric assay for a novel dopamine agonist (+)-trans-3,4,4A,5,6,10B-hexahydro-4-propyl-2H-naphth(1,2-b)(1,4)oxazin-9-ol in human plasma

✍ D. G. Musson; R. J. Tobias Jr; B. K. Maglietto; H. G. Ramjit; W. F. Bayne 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 351 KB 👁 2 views

Development and validation of a sensitiv

Development and validation of a sensitive LC-MS/MS method with electrospray ionization for quantitation of rhein in human plasma: application to a pharmacokinetic study

✍ Buddhadev Layek; T. Santosh Kumar; Ravi Kumar Trivedi; Ramesh Mullangi; Nuggehal 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 305 KB 👁 2 views

Highly sensitive method for the determin

Highly sensitive method for the determination of JI-101, a multi-kinase inhibitor in human plasma and urine by LC-MS/MS-ESI: method validation and application to a clinical pharmacokinetic study

✍ Sandeep Sharma; Naveen Kumar Dubey; Ashish K. Dasgupta; Monashis Sahu; Biju Benj 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 491 KB 👁 3 views

Validated method for determination of br

Validated method for determination of bromopride in human plasma by liquid chromatography–electrospray tandem mass spectrometry: application to the bioequivalence study

✍ P. Nazare; P. Massaroti; L. F. Duarte; D. R. Campos; M. A. M. Marchioretto; G. B 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 143 KB

## Abstract A simple, sensitive and specific liquid chromatography‐tandem mass spectrometry method for the quantification of bromopride I in human plasma is presented. Sample preparation consisted of the addition of procainamide II as the internal standard, liquid‐liquid extraction in alkaline cond