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Development and validation of an enzyme-linked immunosorbent assay for vitellogenin in Chinese loach (Misgurnus angaillicaudatus)

โœ Scribed by Jing Shao; Guoqing Shi; Maoyong Song; Guibin Jiang

Book ID
116447094
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
238 KB
Volume
31
Category
Article
ISSN
0160-4120

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Development and validation of an enzyme-
Development and validation of an enzyme-linked immunosorbent assay to measure vitellogenin in the zebrafish (Danio rerio)
โœ Franรงois Brion; Bente M. Nilsen; Janne K. Eidem; Anders Goksรธyr; Jean Marc Porch ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 910 KB

## Abstract In this study, an enzymeโ€linked immunosorbent assay (ELISA) was developed to quantify vitellogenin (Vtg) in zebrafish (__Danio rerio__). Zebrafish Vtg (zfโ€Vtg) was purified from wholeโ€body homogenates of estradiolโ€exposed zebrafish, and polyclonal antibodies against zfโ€Vtg were raised.