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Validation of an enzyme-linked immunosorbent assay for measuring vitellogenin in california halibut (Paralichthys californicus)

✍ Scribed by Celia Vázquez-Boucard; Mario Burgos-Aceves; Fabiola Arcos-Ortega; Gerardo Anguiano-Vega

Book ID
111848516
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
247 KB
Volume
27
Category
Article
ISSN
0730-7268

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Development and validation of an enzyme-
Development and validation of an enzyme-linked immunosorbent assay to measure vitellogenin in the zebrafish (Danio rerio)
✍ François Brion; Bente M. Nilsen; Janne K. Eidem; Anders Goksøyr; Jean Marc Porch 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 910 KB

## Abstract In this study, an enzyme‐linked immunosorbent assay (ELISA) was developed to quantify vitellogenin (Vtg) in zebrafish (__Danio rerio__). Zebrafish Vtg (zf‐Vtg) was purified from whole‐body homogenates of estradiol‐exposed zebrafish, and polyclonal antibodies against zf‐Vtg were raised.