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Arctic charr (Salvelinus alpinus) vitellogenin: development and validation of an enzyme-linked immunosorbent assay

✍ Scribed by Helge K Johnsen; Helge Tveiten; Nils P Willassen; Arne M Arnesen


Book ID
117485999
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
212 KB
Volume
124
Category
Article
ISSN
1096-4959

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Development and validation of an enzyme-
✍ François Brion; Bente M. Nilsen; Janne K. Eidem; Anders Goksøyr; Jean Marc Porch 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 910 KB

## Abstract In this study, an enzyme‐linked immunosorbent assay (ELISA) was developed to quantify vitellogenin (Vtg) in zebrafish (__Danio rerio__). Zebrafish Vtg (zf‐Vtg) was purified from whole‐body homogenates of estradiol‐exposed zebrafish, and polyclonal antibodies against zf‐Vtg were raised.