Development and validation of a particle-enhanced turbidimetric immunoassay for C-reactive protein
β Scribed by Christopher P. Price; Andrew K. Trull; Debbie Berry; Eileen G. Gorman
- Book ID
- 118900622
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 479 KB
- Volume
- 99
- Category
- Article
- ISSN
- 0022-1759
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Polyfunctional hydrophilic microspheres of 125-nm diameter can be produced by copolymerization of acrylic monomers. Purified c-reactive protein (CRP) was covalently bound to these new micropheres, and the conjugate obtained was used as reagent in a microparticle-enhanced nephelometric immunoassay fo
Novel assay techniques were applied to a newly developed microparticle-based assay for C-reactive protein (CRP). By using two different sized microparticles covalently coated with two monoclonal antibodies of different reactivity, high analytical sensitivity and a high upper measuring limit could be