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Deuterated abscisic acid analogs for mass spectrometry and metabolism studies

✍ Scribed by Suzanne R. Abrams; Ken Nelson; Stephen J. Ambrose

Publisher
John Wiley and Sons
Year
2003
Tongue
French
Weight
109 KB
Volume
46
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

Four analogs of abscisic acid (ABA) with deuterium atoms at non‐exchangeable positions have been synthesized to be used as standards for quantitation of the plant hormone ABA by mass spectrometry and also to be employed as substrates for metabolism studies. Deuterium atoms were introduced in the side chain of the molecule, at C‐4 and/or C‐5, by deuteride or hydride reduction of a propargylic alcohol, an intermediate in the synthesis. As well, deuterium labels were introduced at the C‐8β€² position by conjugate addition of a Grignard reagent containing the label to a cyclohexadienone intermediate, affording specific isotopically labeled ABA molecules with one to five deuterium atoms. Copyright Β© 2002 John Wiley & Sons, Ltd.

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