Determination of β2-agonists in hair by gas chromatography/mass spectrometry

✍ Scribed by Polettini, Aldo; Montagna, Maria; Segura, Jordi; de la Torre, Xavier

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 767 KB
• Volume: 31
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/(sici)1096-9888(199601)31:1<47::aid-jms248>3.0.co;2-o

✦ Synopsis

A method is described for the determination of the &-agonists clenbuterol and salbutamol in hair. The method involves washing hair in sodium dodecyl hydrogensulphate solution, chemical digestion of the hair matrix in alkaline medium, solid-phase extraction, derivatization with methylboronic acid and analysis by gas chromatographylelectron impact mass spectrometry in either the selected-ion monitoring or the scan mode. The effects of chemical digestion and of extraction on the recovery of the analytes were evaluated. Derivatization with methylboronic acid was compared with trimethylsilylation for GC/MS analysis of hair extracts, and was found to give mass spectra which showed more structural information with less chemical noise and better sensitivity. The proposed method was tested on real hair samples obtained from guinea pigs treated with growth-promoting doses of clenbuterol and salbutamol. Both compounds could he detected in hair of treated animals.