Reverse-phase. high-performance liquid chromatography was used for the simultaneous determination of retinol, a-tocopherol, lycopene, a-carotene, and &carotene in human plasma. A multiple solvent system of methanol followed by a mixture of metbanol:acetonitrile:chloroform (47:42: 11) provided clear
✦ LIBER ✦
Determination of β-carotene content and vitamin A activity of vegetables by high-performance liquid chromatography and spectrophotometry
✍ Scribed by A.J. Speek; C.R. Temalilwa; J. Schrijver
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 442 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0308-8146
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In this report we describe a modified reverse phase HPLC method that avoids the solvent evaporation step and allows simple and rapid determination of retinol, alpha-tocopherol, alpha-carotene and beta-carotene and achieves complete separation of alpha- and beta-carotene. Retinyl acetate, alpha-tocop
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