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Simultaneous determination of plasma retinol, α-tocopherol, lycopene, α-carotene, and β-carotene by high-performance liquid chromatography

✍ Scribed by Kenneth W. Miller; Nancy A. Lorr; Chung S. Yang

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
495 KB
Volume
138
Category
Article
ISSN
0003-2697

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✦ Synopsis

Reverse-phase. high-performance liquid chromatography was used for the simultaneous determination of retinol, a-tocopherol, lycopene, a-carotene, and &carotene in human plasma. A multiple solvent system of methanol followed by a mixture of metbanol:acetonitrile:chloroform (47:42: 11) provided clear separation of these compounds. With retinyl acetate as an internal standard, standard curves were developed for each compound on the basis of peak area ratios. The coefficient of variation was less than lO?& in all cases within a run. Between-run reproducibiity was within +2 standard deviations. The method required 100 to 200 ~1 of plasma and 16 min of elution time per sample. Stability studies showed plasma samples could be stored at -10°C with occasional freeze-thaw cycles for 3 to 5 weeks. This method should prove useful in clinical and epidemiological work.

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