Simultaneous determination of procainamide and N-acetylprocainamide in plasma by high-performance liquid chromatography

✍ Scribed by A. G. Butterfield; J. K. Cooper; K. K. Midha

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 488 KB
• Volume: 67
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600670630

✦ Synopsis

A sensitive, specific, high-performance liquid chromatographic procedure is described for the simultaneous determination of procainamide and its metabolite, N-acetylprocainamide, in plasma. Basic plasma (2.0 ml), containing pheniramine maleate as an internal standard, is partitioned with methylene dichloride. The organic extract is concentrated to between 0.3 and 0.5 ml, and 100-microliter aliquots are chromatographed on a microparticulate silica gel column using 0.1% acetic acid-20% 0.1 M ammonium acetate in acetonitrile as the mobile phase. With a fixed-wavelength (254-nm) UV detector, both compounds can be quantitated in the 0.1-8.0-microgram/ml of plasma range.