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Determination of Visnagin inAmmi visnagaHairy Root Cultures Using Solid-Phase Extraction and High-Performance Liquid Chromatography

✍ Scribed by László Kursinszki; Jekaterina Troilina; Éva Szõke

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
58 KB
Volume
59
Category
Article
ISSN
0026-265X

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✦ Synopsis

A high-performance liquid chromatographic method was developed for separation of the furochromone fraction and for determination of visnagin in Ammi visnaga hairy root cultures. Lyophilized samples were extracted with chloroform:methanol (1:1, v/v) and purified on solid-phase extraction cartridges. HPLC analyses were performed on a Eurospher 100-C 8 Knauer column and the mobile phase was 29:28:526:417 (v/v/v/v) acetonitrile:tetrahydrofuran:30 mM citric acid (pH 3.0):methanol. Quercetin was used as internal standard. Peaks were identified by addition of authentic standards and/or by diode-array detection.

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