Determination of the interproton distances in amino groups using NMR-NQR double resonance data
β Scribed by S. V. Anferova; V. S. Grechishkin; V. M. Mikhal'kov; G. V. Mozzhukhin
- Book ID
- 112361447
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 259 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0040-5760
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The amount of free e-amino groups of lysine residues in proteins can be determined by a fast and straightforward method based on the use of r9F NMR spectroscopy. Unsubstituted e-amino groups are trifluoroacetylated with the reagent, S-ethyl trifluorothioacetate, in dimethyl sulfoxide solution, and t
## Abstract ^1^H NMR simultaneous editing of ^13^Cβcoupled and ^13^Cβuncoupled methyl protons resonance, using the selection of double quantum coherences by a gradient pulse, was analyzed __in vitro__ and demonstrated __in situ on__ the hindlimb of an exercised rat model postmortem. __In vitro__ ca