✦ LIBER ✦
1H NMR Determination of lactate 13C-enrichment in skeletal muscle: Using a double quantum filter for the simultaneous editing of 13C-coupled and 13C-uncoupled methyl protons resonance
✍ Scribed by Dr. Gilles Bloch; Laurence Jouvensal; Pierre G. Carlier
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 557 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
^1^H NMR simultaneous editing of ^13^C‐coupled and ^13^C‐uncoupled methyl protons resonance, using the selection of double quantum coherences by a gradient pulse, was analyzed in vitro and demonstrated in situ on the hindlimb of an exercised rat model postmortem. In vitro calibration showed agreement with theoretical analysis. High‐resolution NMR of muscle extract confirmed the accuracy of the lactate ^13^C‐enrichment calculated using the in situ NMR data and the calibration factor obtained in vitro.