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Determination of the inherent kinetics of immobilized glucose oxidase using a diffusion cell

โœ Scribed by S. A. M. Stroe-Biezen; J. M. H. Loo; L. J. J. Janssen; F. M. Everaerts

Book ID
104746373
Publisher
Springer
Year
1996
Tongue
English
Weight
654 KB
Volume
15
Category
Article
ISSN
1615-7605

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โœฆ Synopsis

The enzyme glucose oxidase (GO) was covalently Cg.oCg,, immobilized onto a poly(vinyl alcohol) hydrogel, cross-linked with glutardialdehyde and a polyazonium salt. To compare the kinetic parameters of immobilized GO with the known kinetic cg, A Cg, B parameters of soluble GO, the diffusion cell method was used.

Between two compartments, containing solutions with

CGo

different glucose concentrations, a GO-containing hydrogel D~ff membrane was placed. Simultaneous diffusion through and

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