Three configurations based on the principles behind flow injection analysis (FIA) are proposed for the automatic determination of enzymatic activity. The proposed approaches are normal, stopped-flow and open-closed FIA. The comparative study of the methods developed from these approaches allows the
Determination of the enzymatic activity of pectinases from different microorganisms
โ Scribed by A. E. Maiorano; W. Schmidell; Y. Ogaki
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 182 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1573-0972
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โฆ Synopsis
The decrease in viscosity is widely used to estimate the activity of pectinolytic enzymes. This method is shown to be influenced by the production strain and this prevents an accurate comparison between the activities of different microorganisms, especially under different conditions.
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The use of five cholesterol ester hydrolases (CEH), numbered 1 to 5, for the enzymatic determination of total cholesterol of human and rat serum are compared. All CEH gave approximately the same value (no statistical difference) for human serum. However, when rat serum cholesterol was determined, CE