An unsegmented continuous manifold for development of a heterogeneous immunoassay has been developed in which a microfilter located prior to the detector retains the precipitate formed in the immunochemical reaction. The system used to check the performance of the dynamic approach is monoclonal mous
Comparison of different flow injection approaches to the automatic determination of enzymatic activity
β Scribed by J.M. Fernandez-Romero; M.D.Luque de Castro; M. Valcarcel
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 496 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0731-7085
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β¦ Synopsis
Three configurations based on the principles behind flow injection analysis (FIA) are proposed for the automatic determination of enzymatic activity. The proposed approaches are normal, stopped-flow and open-closed FIA. The comparative study of the methods developed from these approaches allows the establishment of the scope of the application of each, with inherent advantages and disadvantages.
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A quick and reliable enzymatic method for the direct determination of salicylate in pharmaceuticals after appropriate dilution is described. The enzyme salicylate hydroxylase was attached directly onto a platinum working electrode of a wall-jet cell coupled with a flow-injection analysis system. The