Flow-through viscometer for use in the automated determination of hydrolytic enzyme activities: Application in protease, amylase, and pectinase assays
✍ Scribed by J. Kuiper; J.A. Roels; M.H.J. Zuidweg
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 647 KB
- Volume
- 90
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A flow-through viscometer is described, developed for application as a sensor in automated analysis. Its essential part is a glass capillary connected to the sample flow circuit with thin-walled rubber tubes at both ends. These tubes separate the fluid to be tested from a hydraulic liquid. This construction ensures the absence of dead space and a minimal test volume The usefulness of the apparatus is demonstrated in the automated assay of protease, amylase, and pectinase activity. Development of a mathematical model describing the enzymic degradation of macromolecules resulted in a reciprocal equation allowing rectilinear presentation of the calibration data. The feasibility of this model was tested by linear regression analysis of the data.