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Determination of the Donnan effect in equilibrium dialysis and a simple method for its correction

✍ Scribed by Peter Suter; Jurg P. Rosenbusch

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 375 KB
Volume: 82
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90139-7

No coin nor oath required. For personal study only.

✦ Synopsis

Radioactive cesium ions have been used as indicators for the unequal distribution of electrolytes in two-compartment systems which contain nondiffusible macroions in one of the compartments. This method allows the precise determination of the Donnan ratio under conditions in which binding studies of small molecules to proteins are carried out. The corrections, which can be performed easily, are required for ligands whose affinities to proteins are low, particularly if electrostatic protein-ligand interactions preclude the use of high concentrations of diffusible ions. This is illustrated by the comparison of the Donnan equilibrium on the binding of succinate and of CTP to aspartate transcarbamylase from E. co/i.

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