Determination of the binding of tetraalkylammonium bromides to gelatin by an ultracentrifuge method
โ Scribed by J. O. Threlkeld; J. J. Burke; D. Puett; A. Cifferi
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1968
- Tongue
- English
- Weight
- 268 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Ultracentrifugation methods have been widely used for the determination of the free fraction of compounds in plasma, especially for lipophilic compounds. To estimate the effect of contaminated proteins in the ''protein-free phase'' fraction, 200 mL of human plasma was separated into three layers by
In principle, equilibrium analytical ultracentrifugation (AU) can be used to quantify the binding stoichiometry and affinity between small-molecule ligands and proteins in aqueous solution. We show here that heteromeric binding constants can be determined using a data-fitting procedure which utilize