Determination of membrane-bound chloroplast RNA by the ethidium bromide fluorometric method
โ Scribed by Ruth Alscher; Andre T. Jagendorf
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 287 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A method is described for the determination of RNA and DNA in plant tissues which depends on the effect of complex formation on the Huorescence of ethidium bromide. Previous methods were found to be inapplicable to the analysis of plant material because of the high activity of ribonuclease in tissue
In the DNA and RNA assays carried out with the use of ethidium bromide in cell and tissue homogenates according to the method of Karsten and Wollenberger (1). Pronase can be replaced to advantage by heparin in the nucleoprotein dissociation step. Furthermore, rhodamine B is used as a standard instea