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Determination of strontium binding to macromolecules

✍ Scribed by Nam-Won Huh; Pola Berkowitz; Richard G. Hiskey; Lee G. Pedersen

Book ID: 102630684
Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 353 KB
Volume: 198
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(91)90445-y

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✦ Synopsis

An equilibrium dialysis technique for determining the binding of strontium to macromolecules is described. The major difficulty to be overcome is that 90Sr has a decay product, 90Y, which is also a beta-emitter. The described protocol is used to determine the Sr binding isotherm to bovine prothrombin fragment 1. The binding is found to be cooperative, somewhat weaker than Ca binding, and to involve approximately nine strontium sites. The stoichiometric equilibrium constants are determined by nonlinear regression. The procedure should be of great utility for many macromolecules that show strontium affinity.

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