Determination of Stokes radii and molecular masses of sodium hyaluronates by Sepharose gel chromatography
β Scribed by Eiji Shimada; Go Matsumura
- Book ID
- 103147833
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 675 KB
- Volume
- 627
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Even-numbered hyaluronate oligosaccharides ranging from di-to hexadecasaccharides were chromatographed on Sephadex G-25, -50 and -75, and their available volumes (K,,) were examined. Log Kay ratios between any two samples of these oligomers were constant for the three Sephadex types used. The relat
A procedure is described for the determination of the Stokes radius of a detergent micelie by gel chromatography. It was observed that different lots of Sepharose -4B can exhibit a wide variation in the permeation of their gel pores. It is shown that this variation is due to differences in their por
Gel-exclusion chromatography coupled with HPLC instrumentation can be used to determine the molecular weight of highly purified sodium hyaluronate in solution. The method is very reproducible, precise, and rapid, and allows molecular weight determinations up to 2 million to be done in the presence o