Determination of stokes radii of hyaluronate oligosaccharides by Sephadex gel chromatography

✍ Scribed by Eiji Shimada; Go Matsumura

• Book ID: 104144503
• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 563 KB
• Volume: 472
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94102-5

✦ Synopsis

Even-numbered hyaluronate oligosaccharides ranging from di-to hexadecasaccharides were chromatographed on Sephadex G-25, -50 and -75, and their available volumes (K,,) were examined.

Log Kay ratios between any two samples of these oligomers were constant for the three Sephadex types used. The relationships and the use of the log K,, of a substance of known size simplified the processes for calculating Stokes radii of hyaluronate oligosaccharides.