โ Scribed by H. Khalaf; J. Steinert
No coin nor oath required. For personal study only.
Aliphatic secondary arnines were derivatized with the non-fluorescent reagent, Sisothiocyanato-1,3-dioxo-2-p-tolyl-2,3dihydro-lH-benz[de]isoquinoline in a simple and fast two step reaction via thioureas to highly fluorescent formamidines. Derivatives were separated by reversed phase liquid chromatography by using acetonitrile/phosphate buffer as the mobile phase. The limits of detection (S: N=3) ranged from 200 to 300fmol (20~1 injected). A method for the determination of dimethylamine, piperidine and diethanolamine in spiked acidic aqueous samples is given, after the decomposition of their Nnitroso derivatives.
๐ SIMILAR VOLUMES
4-(2-carboxypyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole (NBD-Pro), 4-(2-carboxypyrrolidin-1-yl)-7-(N,N-dimethylamino-sulphonyl)-2,1,3 - benzoxadiazole DBD-Pro), 4-(N-1-carboxyethyl-N-methyl)amino-7-nitro-2,1,3-benzoxadiazole NBD-N-Me-Ala), 4-(N-1-carboxyethyl-N-methyl) amino-7-(N,N-dimethylamino-2