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Determination of prednisolone, prednisone, and cortisol in human plasma by high-performance liquid chromatography

✍ Scribed by N.R. Scott; J. Chakraborty; V. Marks

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
229 KB
Volume
108
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described for the measurement of prednisone and prednisolone in human plasma by high-performance liquid chromatography (hplc). An ether/dichloromethane extract (60:40, v/v) of plasma is evaporated to dryness and chromatographed on a 250 x 4.5mm Whatman Partisil column with uv detection at 239 nm. Prednisone, prednisolone cortisol, and the internal standard dexamethasone are satisfactorily resolved with the elution system of water-saturated dichloromethane/ethanol(95:4, v/v). The hplc method can be used for plasma prednisolone concentrations as low as 25 &ml. The values correlate well with those obtained by a radioimmunoassay procedure for prednisolone.

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