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Free malondialdehyde determination in human plasma by high-performance liquid chromatography

✍ Scribed by Claude Largillière; Serge B. Mélancon

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
281 KB
Volume
170
Category
Article
ISSN
0003-2697

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✦ Synopsis

A high-performance liquid chromatographic method was developed for quantification of malondialdehyde (MDA) in human plasma. Deproteinized samples were injected onto a Waters carbohydrate analysis column which was eluted with 20% (v/v) 0.03 M Tris buffer, pH 7.4, in acetonitrile. Peak absorbancy was measured at 267 nm. In contrast to data already published, we did not detect any free MDA in normal human plasma. This suggests that the classical thiobarbituric acid test is not suitable for the determination of MDA in human plasma.

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