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Determination of picogram quantities of zinc in zinc metalloproteins by atomic absorption spectrometry using a graphite furnace atomizer

✍ Scribed by Takahiro Kumamaru; James F. Riordan; Bert L. Vallee

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 560 KB
Volume: 126
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90132-4

No coin nor oath required. For personal study only.

✦ Synopsis

Optimum operating conditions have been determined for the atomization of zinc from metalloproteins in a graphite furnace. Addition of 50 mM ammonium dihydrogen phosphate to the protein and measurement of the integrated absorbance suppresses or eliminates matrix interference effects. Using a 5-~1 sample both the sensitivity and the detection limit are 0.3 ng of Zn/ml, i.e., 1.5 pg of zinc on an absolute basis. For 10 r&ml of zinc in 5-~1 samples of a zinc metalloenzyme, the coefficient of variation is 1.5%. Accuracy has been established by analysis of zinc metalloenzymes of known zinc stoichiometry. The method has been applied successfully to the determination of zinc in several proteins for which zinc stoichiometry had been unknown.

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