Determination of minute amounts of ATP by flow injection analysis using enzyme amplification reactions and fluorescence detection
✍ Scribed by Elo Harald Hansen; Michèle Gundstrup; Helle Steen Mikkelsen
- Book ID
- 119131883
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 709 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0168-1656
No coin nor oath required. For personal study only.
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A flow-mjectlon spectrophotometrx procedure 1s described for the sequential deternunatlon of glucose, fructose and sucrose m a sample solution by usmg s&e and dual lmmobdtzed enzyme reactors m a smgle mamfold Concentrations III the range 10-5-10-3 M are determmed urlth analysis rates of 30-40 determ
Glutamate and glutamine were determined by lummol chemilummescence with flow-injection analysis (FIA) based on immobilized L-glutamate oxidase and glutaminase coupled with peroxidase The laboratory-made flowthrough cell of the detector has a measured volume of only 15 jA The hydrogen peroxide produc