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Determination of midazolam and its α-hydroxy metabolite in plasma by gas chromatography with electron capture detection

✍ Scribed by Heizmann, P. ;von Alten, R.

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
250 KB
Volume
4
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

A sensitive GC‐ECD assay has been developed for the simultaneous determination of midazolam (I) and its α‐hydroxy metabolite (II) in plasma. The assay involves extraction of both compounds into ether at alkaline pH (pH 12), followed by silylation of the α‐hydroxy metabolite with N,O‐bis(trimethylsilyl) trifluoroacetamide (BSTFA). On extracting 0.5 ml of plasma, the sensitivity limits are 4ng/ml for I and 3ng/ml for II. If present, the minor urinary metabolites, the 4‐hydroxy (III) and the α,4‐dihydroxy compound (IV), can also be determined by this method.

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