Determination of malolactic enzyme activity using an immobilized L-lactate oxidase probe
β Scribed by J. M. Salmon
- Publisher
- Springer-Verlag
- Year
- 1991
- Tongue
- English
- Weight
- 376 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0951-208X
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A sensitive flow system for electrochemical determination of the reduced coenzyme NADH was developed using immobilized NADH oxidase from Buciflus lichenifomis. The immobilized enzyme was packed into a 20 mm x 2 mm I.D. mini-column, and the sample volume used was only 5 ~1 per assay, with an assay t