Direct determination of the cephalosporin transforming activity of immobilized cells with use of an enzyme thermistor
✍ Scribed by P. Gemeiner; A. Welwardová; E. Michalková; L. Welward; L. Kurillová; V. S̈tefuca; B. Danielsson; E. Beraneková
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 105 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0141-0229
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Screening and design of immobilized biocatalysts (IMBs) is a time-consuming process. An ideal process should be universal, fast, convenient, precise, and reproducible. Many of these requirements are met by enzymic flow microcalorimeters, also known as enzyme thermistors (ETs) or thermal assay probes
## Ah&act A flow-lqlectlon system was developed to measure hydrogen peronde usmg the lummol-4-mdophenol reation and an mobtied horseradlsh peroxldase flow cell The unmobkation procedure adopted allowed 1706 mg of perondase to be attached covalently to 1 g of controlled-pore glass Hydrogen peroxide