Determination of sub-nanomole amounts of hydrogen peroxide using an immobilized enzyme flow cell. Application to the determination of ethanol
✍ Scribed by Robert W. Marshall; Timothy D. Gibson
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 537 KB
- Volume
- 266
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
Ah&act
A flow-lqlectlon system was developed to measure hydrogen peronde usmg the lummol-4-mdophenol reation and an mobtied horseradlsh peroxldase flow cell The unmobkation procedure adopted allowed 1706 mg of perondase to be attached covalently to 1 g of controlled-pore glass Hydrogen peroxide could be quantltatlvely detected down to a htmg concentratton of 1 x 10-s M, which corresponded to 1 pmol The mcorporation of lmmobdlzed alcohol ondase mto the flow system allowed the determmatlon of ethanol down to the same low levels Keyword Chemdummescence, Plow system, Enzyme reactor, Ethanol, Hydrogen peromde