Determination of LTE4 in Human Urine By Liquid Chromatography Coupled With Ionspray Tandem Mass Spectrometry

✍ Scribed by Wu, Yunhui; Li, Lily Y.-T.; Henion, Jack D.; Krol, George J.

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 578 KB
• Volume: 31
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/(sici)1096-9888(199609)31:9<987::aid-jms382>3.0.co;2-f

✦ Synopsis

A sensitive and specific high-performance liquid chromatographic/ionspray tandem mass spectrometric (LC/ionspray MS/MS) method was developed and validated to quantitate leukotriene E, (LTE,) in human urine. This method involves solid-phase extraction with Empore membrane disks to isolate LTE, and its internal standard, LTE,-d, , from the urine stabilized with antioxidant and metal ion chelating agent. The reconstituted extracts were analyzed by LC/ionspray MS/MS in the selected reaction monitoring (SRM) mode. The assay has a lower level of quantitation (LOQ) of 50 pg m1-l for LTE, based on 5 ml aliquots of urine. The calibration graphs were linear from 50 pg ml-' to 10 ng ml-' for LTE, extracted from urine. The inter-and intra-assay precision (RSD) and accuracy (DEV) did not exceed 11% and 8% at any level of the calibration standards and quality control (QC) samples, respectively. The recovery of LTE, using the Empore disk solid-phase extraction technology was independent of LTE, concentration in human urine. The overall extraction recovery for LTE, was 72% (RSD = 2.14%).